Conference program

Tuesday 27 June, 2023

5:00pm - 6:30pm 

Opening evening reception

Wednesday 28 June, 2023 

9:00am - 9:10am 

Welcome and opening remarks by the organizers 

9:10am - 10:00am

Virgis  Šikšnys, Vilnius University (Keynote lecture)

Exploring and harnessing the diversity of CRISPR-Cas systems 

Session I-a: Class 1 CRISPR-Cas systems 
Chair: Blake Wiedenheft

10:00am - 10:25am


Malcolm White, University of St. Andrews

Mechanistic Diversity of Type III CRISPR Systems

10:25am - 10:55am

Coffee Break

10:55am - 11:15am

Gregor Hagelueken, University of Bonn

Antiviral signaling by a cyclic nucleotide activated CRISPR protease

11:15am - 11:40am

Asma Hatoum-Aslan, University of Illinois at Urbana-Champaign

Mechanisms of Type III-A CRISPR-Cas defense and counterattack by a phage-encoded protein

11:40am - 12:00pm

Raymond Staals, Wageningen University

Type III-B CRISPR-Cas signaling-based cascade of proteolytic cleavages

12:00pm - 1:30pm 

(Lunch & Learn|12:50pm - 1:20pm Arbor Biotechnologies) 

Session I-b: Class 1 CRISPR-Cas systems
Chair: Chunyi Hu 

 1:30pm - 1:50pm 


Gintautas Tamulaitis, Vilnius University

Antiviral signaling by a cyclic nucleotide activates a RelE-like ribonuclease 

1:50pm - 2:15pm

Lennart Randau, University of Marburg

Functional and structural characterization of Type IV-A CRISPR-Cas-mediated CRISPR interference 

2:15pm - 2:35pm  

Ning Jia, Southern University of Science and Technology

Type IV-A CRISPR-Csf complex: assembly, dsDNA targeting and CasDinG recruitment 

2:35pm – 2:55pm  

Ryan Jackson, Utah State University

Structure and Function of Type IV CRISPR systems and their anti-CRISPRs 

2:55pm - 3:25pm  

Coffee Break 

3:25pm - 3:45pm  

Fabienne Benz, Pasteur Institute
In trans adaptation and RNA-guided silencing by a Klebsiella plasmid-encoded type IV-A3 CRISPR-Cas

Session II-a: Class 2 CRISPR-Cas systems
Chair: Irma Querques

 3:45pm - 4:10pm  

Omar Abudayyeh, Massachusetts Institute of Technology

New Molecular Technologies for Genome Editing and Cell Control 

4:10pm - 4:30pm 

Julene Madariaga-Marcos, University of Leipzig

Investigating the R-loop formation by CRISPR-dCas9 with single-molecule ultrafast twist measurements 

4:30pm - 5:00pm

Coffee Break

5:00pm - 5:20pm

Jack Bravo & Grace Nicole Hibshman, University of Texas at Austin

DNA interrogation by PAMless SpRY-Cas9 

5:20pm - 5:40pm 

Yan Zhang, University of Michigan

CRISPR RNA and tracrRNA serve as a regulatory switch for apoCas9-mediated viral memorization in Neisseria 

5:40pm - 6:00pm 

Yibei Xiao, China Pharmaceutical University

How Cas9-Csn2-Cas1-Cas2 supercomplex select prespacer during spacer acquisition 

6:00pm - 7:30pm 


7:30pm - 9:00pm 

Poster session #1

Thursday 29 June, 2023

9:00am - 9:05am 

Opening remarks for Day 2 

Session II-b: Class 2 CRISPR-Cas systems
Chair: Hong Li

 9:05am - 9:30am 


Dipali Sashital, Iowa State University

Metal-dependent specificity switching in CRISPR-Cas adaptation and interference 

9:30am - 9:50am 

Dina Grohmann, University of Regensburg

Allosteric activation of CRISPR-Cas12a requires the concerted movement of the bridge helix and helix 1 of the RuvC II domain 

9:50am - 10:15am 

Ekaterina Semenova, Rutgers University

tRNA anticodon cleavage by target-activated CRISPR-Cas13a effector 

10:15am - 10:45am 

Coffee Break 

Session III-a: CRISPR-Derived technologies
Chair: Gil Westmeyer 

10:45am - 11:10am 

Kira Makarova, National Institutes of Health

Comprehensive survey of novel CRISPR systems by mining the expansive genomic diversity of prokaryotic organisms 

11:10am - 11:30am 

Ailong Ke, Cornell University

Genome Editing Applications Based on the IscB-ωRNA structure 

11:30am - 11:50am 

Osamu Nureki, University of Tokyo

Minimal and most efficient genome editing Cas effector 

11:50am - 12:10pm 

Chunlei Jiao, Helmholtz Institute for RNA-based Infection Research

TracrRNA reprogramming enables direct, PAM-free detection of RNA with diverse DNA-targeting Cas12 nucleases 

12:10pm - 1:30pm   

(Lunch & Learn|1:00pm - 1:30pm Eli Lilly)

1:30pm - 3:00pm  

Poster session #2

Session III-b: CRISPR-derived technologies
Chair: Patrick Pausch

3:00pm - 3:20pm 

Adrian M. Molina Vargas, University of Rochester
New design strategies for ultraspecific Cas13a-based RNA-diagnostic tools with single-nucleotide mismatch sensitivity

3:20pm - 3:45pm 

Samuel Sternberg, Columbia University

Molecular innovation at the interface between transposons and CRISPR-Cas systems 

 3:45pm - 4:05pm 

Morayma Temoche-Diaz, Metagenomi

Genome editing using novel reverse transcriptases discovered from metagenomics 

4:05pm - 4:25pm 

David Schwark, Pairwise

A Novel Mechanistic Framework for Precise Sequence Replacement Using Reverse Transcriptase and Diverse CRISPR-Cas Systems 

4:25pm - 4:55pm 

Coffee Break 

Session IV-a: CRISPR ecology and evolution
Chair: Rolf Backofen

 4:55pm – 5:15 pm 


Raul Perez-Jimenez, Center for Cooperative Research in Biosciences

Evolution of CRISPR-associated endonucleases as inferred from resurrected proteins 

5:15pm - 5:40pm 

Lotta-Riina Sundberg, University of Jyväskylä

Mucin induces CRISPR-Cas defense in an opportunistic pathogen 

5:40pm - 6:00pm 

Harry Edwards, University of Nottingham

Linking the SOS response and CRISPR Cas adaptation 

7:00pm - 10:00pm 

Friday 30 June, 2023

9:00am - 9:05am 

Opening remarks for Day 3 

Session IV-b: CRISPR ecology and evolution
Chair: Wolfgang Hess

 9:05am - 9:25am 


Owen Tuck, University of California Berkeley

Genome expansion by a CRISPR trimmer-integrase 

9:25am - 9:45am  

Nathaniel Burman, Montana State University

Type III CRISPR-mediated Control of a Toxin-Antitoxin System 

9:45am - 10:10am 

Stineke van Houte, University of Exeter

Understanding ecological determinants of CRISPR-phage interactions 

10:10am - 10:30am 

Hélène Chabas, ETH Zurich

CRISPR-Cas systems: a barrier for antibiotic-resistant conjugative plasmids? 

10:30am - 11:00am 

Coffee Break 

11:00am – 11:20am 

Reetta Penttinen, University of Jyväskylä

CRISPR-harboring megaphages isolated from boreal freshwater environment 

Session V-a: Beyond CRISPR
Chair: Alan Davidson

 11:20am - 11:40am 


Joshua Modell, Johns Hopkins University School of Medicine 

Prophages vaccinate CRISPR-Cas populations against lytic threats


11:40am - 12:05am 

Joseph Bondy-Denomy, University of California San Francisco
Anti-CRISPR proteins as mediators of phage-host symbiosis

12:05pm - 1:25pm


1:25pm - 2:30pm 

Poster session #3

2:30am - 2:50pm 

Kevin Forsberg, University of Texas Southwestern Medical Center

The mysterious origins of anti-CRISPR proteins 

2:50pm - 3:15pm 

Peter Fineran, University of Otago

Regulation of anti-CRISPR production 

3:15pm - 3:35pm 

Alexander Meeske, University of Washington

Viral cas genes antagonize CRISPR immunity 

3:35pm - 4:05pm 

Coffee Break 

Session V-b: Beyond CRISPR
Chair: John van der Oost

 4:05pm - 4:30pm 


Rotem Sorek, Weizmann Institute

Phages overcome bacterial immunity via diverse anti-defense proteins 

4:30pm - 4:50pm 

Giuseppina Mariano, University of Surrey 

The novel anti-phage system Shield co-opts an RmuC domain to mediate phage defense across Pseudomonas species 

 4:50pm – 5:15pm 

David Bikard, Pasteur Institute

An anti-anti-CRISPR system: triggering cell death in response to phage counter-defenses 

5:15pm – 5:35pm 

Daan Swarts, Wageningen University

The diverse functions and mechanisms of prokaryotic Argonaute proteins 

5:35pm - 5:50pm  

Closing remarks and announcement of CRISPR2024 in New Zealand 

7:00pm - 11:00pm 

Conference Dinner with wine tasting in the Würzburg Residenz wine cellar 

Saturday 1 July, 2023

 FUN DAY in Würzburg 

9:30am - 12:30pm 

12:30pm - open end